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AntiFKHR Antibody (C9) is a mouse monoclonal IgG 1 κ FKHR antibody, cited in 30 publications, provided at 0 µg/ml;The PAX3–FKHR and PAX7–FKHR proteins are capable of recognizing PAX3/PAX7 DNAbinding sites and regulating the expression of genes containing these sites When compared to the transcriptional activity of wildtype PAX proteins, PAX3–FKHR and PAX7–FKHR fusion proteins have enhanced transcriptional activityThis test is a FISH analysis performed on tumor specimens and identifies rearrangement (translocation) of the FOXO1 gene (also known as the FKHR gene) resulting in gene fusion with PAX3 or PAX7 gene, which is observed in alveolar subtype of rhabdomyosarcoma Presence/absence of this gene fusion can aid in differentiation of rhabdomyosarcoma

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Fakhri-Pax3/FKHR is a more potent activator of MET than Pax3 Expression of Pax3 in the human osteosarcoma cell line, SaOS2, causes a MET (Wiggan and Hamel, 02;The FKHR regulatory domain functions at a much higher rate than the normal PAX3 regulatory domain 6, 7 The increased activity of the PAX3/FKHR transcription factor is associated with the dysregulation of myogenic lineage, resulting in malignant transformation 8, 9

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A FKHR gene rearrangement is found in alveolar rhabdomyosarcoma (ARMS) ARMS is characterized by two pathognomonic translocations which generate gene fusions t(2;13)(q35;q14) PAX3FKHR and t(1;13)(p36;q14) PAX7FKHR, found in 80% and 15% of cases, respectivelyFOXO1 (Forkhead Box O1) is a Protein Coding gene Diseases associated with FOXO1 include Rhabdomyosarcoma 2 and GliomaAmong its related pathways are Common Cytokine Receptor GammaChain Family Signaling Pathways and Transcriptional misregulation in cancerGene Ontology (GO) annotations related to this gene include DNAbinding transcription factor activity and chromatin bindingA few weeks later, FKHR thought it is now safe to put his head above the parapet and put his money where his mouth is by documenting his own 5* Seox grind on Twitter Allegations soon surfaced that FKHR's ingame rank increased by a rather unrealistic amount during the brief attempt, leading some commentators to question whether FKHR has been

Plasmid 1098 pcDNA GFP FKHR from Dr William Sellers's lab contains the insert FKHR and is published in Mol Cell Biol 00 Dec (23)69 This plasmid is available through AddgeneBackground FoxO1/FKHR FoxO1, also called FKHR (forkhead in ­rhabdomyosarcoma), is a 655 amino acid (aa), 70 kDa, ubiquitously expressed member of the forkhead box O family of winged helix transcription factors In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferationTwo cell lines, RD and HX170C, were stably transfected with a PAX3FKHR expression construct In cloned transfectants derived from both lines, PAX3FKHR expression resulted in increased proliferative rate in vitro and promoted cell growth in the absence of added growth factors

There were 37 tumours with t(2;13)/PAX3FKHR, 8 with The quality of RNA extracted from each fixed tumour sample was t(1;13)/PAX7FKHR and 46 with neither (Table 1) Overall, more deemed to be informative for analysis of its PAX3/7FKHR status than one methodology was used in 45 casesFKHR belongs to the superfamily of forkhead transcription factors (FOX) It is characterized by a common Forkhead (or winged helix) domain, a variant of the helixturnhelix motif FKHR contains three residues, Thr24, Ser256, and Ser319 that lie within consensus sequences for phosphorylation by protein kinase B (PKB)FKHR, FKHRL1, and AFX sense and antisense probes were produced by transcription from the T 3 and T 7 promoters using NotI and SpeI digested vector, respectively Each slide was incubated in 80 μl of hybridization solution containing 5 million counts of the appropriate probe overnight at 55 C in a humid chamber

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FKHR belongs to the superfamily of forkhead transcription factors (FOX) It is characterized by a common Forkhead (or winged helix) domain, a variant of the helixturnhelix motif FKHR contains three residues, Thr24, Ser256, and Ser319 that lie within consensus sequences for phosphorylation by protein kinase B (PKB)The rearrangement causes the PAX3 gene to be fused with the FOXO1A gene (also called FKHR) on chromosome 13 The fused PAX3FOXO1A gene has an increased ability to activate genes involved in myogenesis and can prevent cell death As a result, muscle cell growth becomes uncontrolled, which can lead to this cancer of muscle tissueThe FKHR gene was first identified from its disruption by the t(2;13) chromosomal translocation seen in the pediatric tumor alveolar rhabdomyosarcoma It encodes for a member of the forkhead family of transcription factors Recently, a homolog of FKHR in the nematode Caenorhabditis elegans was identified called DAF16, which is a downstream target of two Akt homologs in an insulinrelated

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We now report on the role of forkhead homologue in rhabdomyosarcoma (FKHR), a recently described member of the forkhead/wingedhelix transcription factor family, as a mediator of endometrial differentiation Sustained 8BrcAMP stimulation resulted in the induction and nuclear accumulation of FKHR in differentiating ES cellsFKHR Antibody (C9) is recommended for detection of FKHR of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA;FKHR belongs to the superfamily of forkhead transcription factors (FOX) It is characterized by a common Forkhead (or winged helix) domain, a variant of the helixturnhelix motif FKHR contains three residues, Thr24, Ser256, and Ser319 that lie within consensus sequences for phosphorylation by protein kinase B (PKB)

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TransAM ® FKHR (FOXO1) dnabinding ELISA for activated FKHR (FOXO1) transcription factor TransAM ® Kits are DNAbinding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts Assays are available for over 40 different targets (see the list at right)We now report on the role of forkhead homologue in rhabdomyosarcoma (FKHR), a recently described member of the forkhead/wingedhelix transcription factor family, as a mediator of endometrial differentiation Sustained 8BrcAMP stimulation resulted in the induction and nuclear accumulation of FKHR in differentiating ES cellsActivation of the transcription factor FKHR (F or k head in h uman r habdomyosarcoma, FOXO1a) in various established cell lines induces cell cycle arrest followed by apoptosisThese effects are inhibited through activation of the phosphatidylinositol 3‐kinase/Akt pathway, resulting in FKHR phosphorylation and its export from the nucleus, thus blocking its pro‐apoptotic activity

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1 Neurobiol Dis 14 Sep; doi /jnbd Epub 14 May 27 Activation of Akt/FKHR in the medulla oblongata contributes to spontaneous respiratory recovery after incomplete spinal cord injury in adult ratsWiggan et al, 02)During thisFoxO1/FKHR Products FoxO1, also called FKHR (forkhead in rhabdomyosarcoma), is a ubiquitously expressed member of the forkhead box O family of winged helix transcription factors In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferation

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FOXO1 (FKHR) (13q14) Gene Rearrangement by FISH Disease Overview Incidence – rhabdomyosarcomas are the most common softtissue sarcoma in childrenEntry name i V9H0B8_HUMAN Accession i V9H0B8 Primary (citable) accession number V9H0B8 Entry history i Integrated into UniProtKB/TrEMBL February 19, 14 Last sequence update February 19, 14 Last modified December 11, 19 This is version 12 of the entry and version 1 of the sequence See complete history Entry status i Unreviewed (UniProtKB/TrEMBL) Disclaimer AnyEntry name i V9H0B8_HUMAN Accession i V9H0B8 Primary (citable) accession number V9H0B8 Entry history i Integrated into UniProtKB/TrEMBL February 19, 14 Last sequence update February 19, 14 Last modified December 11, 19 This is version 12 of the entry and version 1 of the sequence See complete history Entry status i Unreviewed (UniProtKB/TrEMBL) Disclaimer Any

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Furthermore, FKHR and C/EBPbeta were shown to interact directly in a glutathione Stransferase pulldown assay These results provide the first evidence of regulated expression of FKHR and demonstrate that FKHR has an integral role in PKAdependent endometrial differentiation through its ability to bind and functionally cooperate with C/EBPbetaPlasmid pcDNA3 Flag FKHR from Dr Kunliang Guan's lab contains the insert FKHR and is published in J Biol Chem 1999 Jun 11 274(24) This plasmid is available through AddgenePurification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phosphopeptide and nonphosphopeptide affinity columns Applications WB IHC IF Species Reactivity Hu Ms Rt Specificity The antibody detects endogenous level of FKHR only when phosphorylated at serine 256

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FKHR / Platinum 4 23LP / 95W 79L Win Ratio 55% / Xerath 13W 11L Win Ratio 54%, Maokai 12W 2L Win Ratio 86%, Karma 7W 6L Win Ratio 54%, Seraphine 6W 5L Win Ratio 55%, Senna 6W 4L Win Ratio 60%Abstract Rhabdomyosarcoma is a family of myogenic soft tissue tumors subdivided into two main subtypes alveolar (ARMS) and embryonal (ERMS) ARMS is characterized by a frequent 2;13 chromosomal translocation that creates a PAX3‐FKHR fusion transcription factorWestern Blot FoxO1/FKHR Antibody (N7F8) NBP Analysis of (A) Ntera2 cell lysate, (B) HEK293 cell lysate, and (C) human placenta tissue using FOXO1A antibody at a concentration of 2 ug/ml

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FoxO1/FKHR Products FoxO1, also called FKHR (forkhead in rhabdomyosarcoma), is a ubiquitously expressed member of the forkhead box O family of winged helix transcription factors In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferationThe PAX3FKHR fusion protein is present in a majority of alveolar rhabdomyosarcomas associated with increased aggressiveness and poor prognosis To better understand the molecular pathogenesis of PAX3FKHR, we carried out the first, unbiased genomewide identification of PAX3FKHR binding sites and associated target genes in alveolar rhabdomyosarcomaKimura Yuito (KMR) and Fukuhara Tetsuya (FKHR), the producer and director of Granblue Fantasy, talk about GBF 's increasing international presence, and provide a behindthescenes look at the fun side of GBF development Continued from Part 1

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Alveolar rhabdomyosarcoma (ARMS) is a subtype of the rhabdomyosarcoma soft tissue cancer family whose lineage is from mesenchymal cells and are related to skeletal muscle cells ARMS tumors resemble the alveolar tissue in the lungs Tumor location varies from patient to patient, but is commonly found in the head and neck region, male and female urogenital tracts, the torso, and extremitiesThe latest tweets from @hiyopiAlveolar rhabdomyosarcomas (ARMS) are aggressive softtissue sarcomas affecting children and young adults Most ARMS tumors express the PAX3FKHR or PAX7FKHR ( PAXFKHR ) fusion genes resulting from the t(2;13) or t(1;13) chromosomal translocations, respectively However, up to 25% of ARMS tumors are fusion negative, making it unclear whether ARMS represent a single disease or multiple

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Genetic alterations of FKHR (FOXO1), AF6q21 (FOXO2), and AFX (FOXO4), closely related members of the forkhead family of DNA binding proteins, in human cancers has suggested they play a role in the regulation of cellular differentiation or proliferationLo, "Expression of FKHR, FKHRL1, and AFX genes in the rodent ovary evidence for regulation by IGFI, estrogen, and the gonadotropins," Molecular Endocrinology, vol Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2Background FoxO1/FKHR FoxO1, also called FKHR (forkhead in ­rhabdomyosarcoma), is a 655 amino acid (aa), 70 kDa, ubiquitously expressed member of the forkhead box O family of winged helix transcription factors In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferation

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FOXO1/FKHR (fork head in Rhabdomyosarcoma) FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNAbinding domain known as the Forkhead Box, or FOX The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,The PAX3–FKHR and PAX7–FKHR proteins are capable of recognizing PAX3/PAX7 DNAbinding sites and regulating the expression of genes containing these sites When compared to the transcriptional activity of wildtype PAX proteins, PAX3–FKHR and PAX7–FKHR fusion proteins have enhanced transcriptional activityTo identify downstream targets of PAX3‐FKHR, we introduced an inducible form of PAX3‐FKHR into human RD ERMS cells Microarray analysis identified 39 genes (29 upregulated and 10 downregulated) that are modulated by PAX3‐FKHR in RD cells and differentially expressed between ERMS and PAX3‐FKHR‐positive ARMS tumors

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FKHR, FKHRL1, and AFX sense and antisense probes were produced by transcription from the T 3 and T 7 promoters using NotI and SpeI digested vector, respectively Each slide was incubated in 80 μl of hybridization solution containing 5 million counts of the appropriate probe overnight at 55 C in a humid chamberFKHR (FOXO1) Transcription Factor Info The Forkhead transcription factor FKHR (also known as FOXO1, or Forkhead in rhabdomyosarcoma) belongs to the FOX family of transcription factors that regulate genes involved with proliferation, differentiation and longevityWhile all FOX proteins contain the distinct forkheadbox motif that binds DNA, the proteins can be further grouped into subclassesPlasmid pcDNA3 Flag FKHR from Dr Kunliang Guan's lab contains the insert FKHR and is published in J Biol Chem 1999 Jun 11 274(24) This plasmid is available through Addgene

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FKHR Lentiviral Activation Particles (h) contain the following SAM Activation elements a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2p65HSF1 fusion protein and a targetspecific nt guide RNA They also contain the blasticidin, hygromycin and puromycin resistance genesThe resulting chimeric protein PAX3FKHR is a potent transcriptional activator, and is hypothesized to function as a dominant acting oncogene To investigate its biological function, PAX3FKHR wasForkhead box protein O1 (FOXO1) also known as forkhead in rhabdomyosarcoma (FKHR) is a protein that in humans is encoded by the FOXO1 gene

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